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KMID : 0378019760190020165
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New Medical Journal
1976 Volume.19 No. 2 p.165 ~ p.171
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Two Distinctive Population of Lymphocytes in Immunized Leghorn Distinguishable by Immunofluorescence
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Abstract
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In view of the increasing evidence for the heterogeneity of lymphocytes and the importance of this heterogeneity to the immune response, there is a pressing need for physiological markers to distinguish one type of lymphocyte from another. Many procedures are currently in use to determine B cells. There are Ig staining by fluorescence determination of the C2 receptor by sheep cells with mouse or human complement, determination of the Fc receptor by uptake of aggregated ?-globulin and determination of the mouse specific B lymphocyte antigen by the dye exclusion cytotoxic test with anti MBLA. Thymus-specific antigens, such as TL, RTA and ? have also been discovered. This study was made to distinguish B cells from T cells in the lymphoid tissues of immunized Leghorn. Rabbit antithymic lymphocyte sera were prepared by immunizing rabbit with Leghorn thymocytes and rabbit antibursal lymphocytes were prepared by immunizing rabbit with the bursal lymphocytes, hereinafter referred to as ATLS and ABLS, respectively. For preserving specificity selective only to thymic and bursal lymphocytes ATLS and ABLS were cross absorbed with thymic or bursal lymphocytes. For antigenic stimulation 1 ml of horse serum was injected intravenously and the chicken were boosted in the same manner at 1 week. ATLS and ABLS were conjugated with fluorescein isothiocyanate after precipitation of 34 percent saturated ammonium sulfate, and then fractionated on DEAE cellulose respectively. The animals were bled at 3 days, 10 days and 20 days after the last injection and Bursa of Fabricius, Thymus, Spleen and Cecum were examined. The result were as follows 1. The antibursa lymphocyte antiserum and antithymus lymphocyte antiserum were specific to B lymphocytes and T lymphocytes in the lymphoid tissues by immunofluorescent technique. 2. In the splenic tissues B cells or group of B cells were seen collectively in the lymph follicles and distributed widely in the red pulp and sinuses. 3. In the cecal tissues B cells were seen collectively in the lymph follicles and distributed widely in the mucosal tissues. And T cells were seen widely in the mucosal tissues.
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KEYWORD
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